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聚合酶链反应技术
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聚合酶链反应技术
Encyclopedia
工学
刑事科学技术
释
polymerase chain reaction; PCR
聚合酶链反应技术
利用体外模拟生物体细胞分裂DNA复制过程,双链目标DNA在体外模拟生理环境中(包括适当浓度的成对引物,dNTP, Tag酶,缓冲溶液),经由变性、退火、延伸三个步骤反复循环进行,得到与目标DNA相同的大量拷贝,快速扩增特定基因或DNA序列的技术。是第二代DNA分析技术,又称PCR技术。
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