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无细胞蛋白质合成系统
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无细胞蛋白质合成系统
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理学
基因工程
释
cell-free protein synthesis system
无细胞蛋白质合成系统
以细胞提取物为基础,以外源DNA或信使RNA(mRNA)为模板,利用细胞抽提物中的蛋白质因子、相关酶系等,通过在体系内补充转录和翻译所需的底物(如腺苷三磷酸、鸟苷三磷酸、氨基酸及能量再生物质等),实现目标蛋白质的体外表达合成的体外蛋白质合成方式。又称无细胞蛋白质表达系统或无细胞表达体系。
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