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斑点和狭缝印迹杂交
释义
斑点和狭缝印迹杂交
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理学
分子杂交
释
dot and slot blotting
斑点和狭缝印迹杂交
将待测核酸直接点在膜上或通过点样器抽滤到膜上,经碱变性、中和后,DNA单链与膜结合成圆形斑点印记与探针杂交的方法称为斑点印迹杂交。根据相同原理发展出的狭缝式点样器,杂交信号是条带而不是圆点,称为狭缝印迹杂交。
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