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快速低温变性共扩增聚合酶链反应
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快速低温变性共扩增聚合酶链反应
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理学
连接酶链式反应
释
fast co-amplification at lower denaturation temperature-PCR,COLD-PCR
快速低温变性共扩增聚合酶链反应
利用双链DNA合成时由单个核苷酸错配引起该段序列的熔解温度产生微小和可预测的变化,设置合适的熔解温度进行聚合酶链反应(PCR),从而放大突变点所在链的扩增效率的技术。
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