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完全低温变性共扩增聚合酶链反应
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完全低温变性共扩增聚合酶链反应
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理学
连接酶链式反应
释
full co-amplification at lower denaturation temperature-PCR,COLD-PCR
完全低温变性共扩增聚合酶链反应
利用双链DNA合成时由单个核苷酸错配引起该段序列的熔解温度产生的微小和可预测的变化,优先扩增和识别少数等位基因和低水平体细胞DNA突变,使之更容易被检测的方法。
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