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全内反射荧光显微术
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全内反射荧光显微术
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total internal reflection fluorescence microscopy; TIRFM
全内反射荧光显微术
通过利用全内反射产生的消逝波进行激发,将样品处激发区域限制在玻片表面100纳米左右范围以内,从而能够很大程度上减少背景荧光干扰,增强信噪比的荧光显微成像技术。
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