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RNA连接酶介导的5′端快速扩增
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RNA连接酶介导的5′端快速扩增
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理学
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RNA ligase-mediated rapid amplification of 5′ends,RLM-5′RACE
RNA连接酶介导的5′端快速扩增
针对截断的互补DNA(cDNA)的产生而进行改良的末端快速扩增法。在cDNA合成之前将寡核苷酸连接到信使(mRNA)的5′端和3′端,使得多聚腺苷酸尾的长度得以确定,同时也避免了反转录提前终止的问题。
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